In order to understand who Yamnaya people were, one must first define ‘Yamnaya’. We will adopt a strictu sensu view (e.g. Anthony, Heyd) encompassing burials dating 3200-2600 BC, with a characteristic body position, mound construction, and copper artefacts. These complexes can be linked to a core group of people whose autosomal make-up is quite homogeneous throughout their wide geographic range. Moreover, almost all males belong to Y-haplogroup R1b-M269-Z2103. In this light, ‘core Yamnaya’ does not represent a ‘proto-Indo-European’ population, as commonly proclaimed, but a group which contributed to several post-PIE population-language complexes, such as Tocharian, Armenian and some Paleo-Balkan languages. However, historical linguistics is not the focus of this post. Archeologists had linked Yamnaya to earlier complexes such as Khvalynsk, Repin and/or Mikhalivka. Given that cultural markers such as pottery and burial customs can be borrowed and copied, ancient DNA can offer a more objective assessment of population origins. However, the cacophony of clusters, clines and other statistical constructs in publications can be confusing. A more rationalized approach is required, and one way is to co-analyse phylogenetically linked individuals across space and time. Apart from a lower-quality individual from Smyadovo (Bulgaria c. 4300 BC), the earliest attestation of R1b-M269 is in two individuals from the Kuban steppe (Stavropol region) c. 3700 BC -NV3003 and KST001 (Ghaliachi et al 2024). However, Y-hg R1b-M269 is missing in currently sampled Kuban steppe and north Caucasian males from the preceding period (5000-4000 BC). Males of the ‘Kuban steppe 4500bc’ group (Progress, Vojnucka, Sengeleevskiy, etc) are instead derived for the phylogenetically divergent Y-hg Rb-V1636. Males from the Nalchik cemetery are also derived for Y-hg R1b-V1636, or related haplotypes, although they were buried in a ‘Caucasian Farmer’ pose and heavily infused with such ancestry, but probably also had a burial mound thrown above. We do not know when the R1b-V1636 clans entered the northern Caucasus region, or from where, but they appear to have been attracted by trade with North Caucasian Famer (~Eneolithic) groups- termed as ‘Meshoko-Zamok’, ‘Chokh’, etc, in literature. Curiously the Nalchik group has minimal Central Asian (“TTK-related”) ancestry, whilst the Kuban steppe group has high levels. This suggests that TTK-related ancestry arrived after R1b-V1636 dominant EHG clans entered the North Caucasus region, but other scenarios are possible. Lastly, two ‘Meshoko culture’ males from Unakozovskaya have been assigned to Y-hg J2a-L26. A shake-up occurred in the north Caucasus after 4000 BC. As we know, this corresponds to the emergence of the Majkop phenomenon, catalysed by renewed migrations from the south. These were not ‘Uruk migrants’ as sometimes proposed - the Uruk phenomenon occurred several hundred years later and was a south Mesopotamian phenomenon. Instead, these newcomers emerge from southern Caucasus- north Mesopotamian ‘Late Chalcolithic’ groups. They brought with them multiple West Asian lineages, such as Y-hg T, L2, J2a-, J2b, G2. Over time they mixed with preceding north Caucasian Eneolithic groups, culminating in the Novosvobodnaja phenomenon. The emergence of Majkop as a new socio-cultural complex broke down the previous system dominated by Y-hg R1b-V1636 clans. The Majkop sphere consisted of a ‘core’ of heterarchical chiefs buried in elaborate kurgans near the Mountains, and a dynamic northern ‘frontier’ in the steppe lands (as far as the lower Don) between 4000 and 3000 BC. At least 3 ‘‘Majkop periphery’ genetic groups can be defined; in fact all these groups can be termed ‘steppe Majkop’: 1- Group with western Siberian/ north central Asian ancestry (the ‘genetic steppe Majkop’ as defined in Wang et al, 2023) 2- The South Caucasian/north Iranian ‘Zolotarevka’ group 3- The R1b-M269 duo. Regardless of their lineages and genomic affinities, these individuals were often buried in kurgans which over time formed groups. These were not continuations of pre-4000 BC kurgans, but the communities instead made a conscious choice to build new kurgans after 4000 BC, adding to the idea of discontinuity. But once built, these kurgan clusters continued to be developed for hundreds of years, into the Yamnaya period. This does imply ethnic homogeneity or continuity, just a ‘continuity of place’. Without a direct attestation of a phylogenetic ancestor, and guestimating from their (non-identical) genomic profile, we are left to speculate that Y-hg R1b-M269 individuals moved down from somewhere in the Volga-Don interfluve. Perhaps amongst groups utilizing Repin pottery, but if so, they did not continue its use in their new contexts. By 3000 BC, the Majkop system collapsed. Yamnaya groups and their ‘Catacomb’ descendants took control of the north Caucasus region, having benefitted from years of trade/ exchange and knowledge gathering. Whether Yamnaya actually descend from individuals like KST-1 or NV3003 remains to be seen, however these are the closest leads we have. Certainly, we can model Yamnaya as deriving from KST-1 (88%) + Dnieper_N (12%), but we should be cautious when using singular individuals as ‘sources’.See also... The PIE homeland controversy: December 2024 open thread
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Sunday, June 22, 2025
‘Proto-Yamnaya’ Eneolithic individuals from Kuban steppe c. 3700 BC ? (guest post)
This is a guest post by an anonymous contributor. I don't necessarily agree with its findings, but I think it's a good way to get the ball rolling here again. Feel free to let me know what you think. Please note, however, that any comments that show mental instability will be blocked. No more crazy talk on this blog.
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40 comments:
Great post, good to see the ball rolling again!
The post title should read "3700 BC", not "37000".
It's mostly fairly well written but much of what is said was already known. It can basically be reduced to "we still don't know a lot of details due to a paucity of samples". For instance, we already knew that NV3003 is the oldest sample with a high number of R-M269 phylogenetic equivalents but it is also late since it from about 3700 BC and is ancestral for R-L23 and for Z2103. The path for Z2013 is M269>L23>Z2103, which the author neglected to point out, and R-L23 is from about 4350 BC. So M269>L23 was in existence for about 650 years and Z2103 was in existence for about 500 years before NV3003. The coverage of KST001 is too low to determine whether it had a lot of derived M269 or if it was derived for L23 or not. There is still too much that we don't know about how L23 and Z2103 became involved because we still don't have specimens with a lot of M269 equivalents from 4500BC-4300BC and that is probably due to R-M269 having been a small population evidenced by the large number of phylogenetic equivalents. We keep hoping for a miracle that those specimens will be found. Obviously this is only one portion of the problem.
KST001
Golubaya_Krinitsa
Tutkaul1
Azerbaijan_Caucasus_lowlands_LN
best coefficients: 0.724 0.152 0.124
tail prob 0.2729
Here is a model for KST001. Clearly demontrates PIA came from the Don region. Golubaya_Krinitsa is a mix of ukraine mesolithic and the well known R1b Samara HG
I agree that KST001(more Yamnaya-like) and NV3003 (more Piedmont-like) being R-M269 really make it look like proto-Yamnaya was somewhere marinating around the Don at this time.
The lower Don doesn't seem like a great candidate, given how much excess UKR_N those "Don Yamnaya" samples have. So I further agree it may have something to do with Repin.
here is a post courtesy of genrachivist
Don_EBA_Yamnaya = 97% SS_high + 3% Alkhantepe/Maikop
Rest of Yamnaya = 66-80% SS_high + 20-34% Remontnoye
So basically Don_EBA_Yamnaya is ancestral to the Rest of Yamnaya Cluster. The earliest Yamnaya profile was Don_EBA
Highest Remontnoye (34%) is in CaspianInland_EBA_Yamnaya
I think it’s good to get a summary of what’s known, broken down in digestible subtopics. ‘We’ might long know about these samples, but other people do not, and how they fit within the broader scheme of things even less so.
Another interesting theme that emerges here is that even after the Satanay cave - EHG sample published by Ghaliachi, all these samples with high CHG & low / absent Cauc Farmer ancestry lend support the mid-northern CHG niche theory.
(Lower) Don Yamnaya has a mid 4th millennium BC formation as per DATES, suggesting it being a mixture of something more UKR_N-rich and incoming Yamnaya (which actually has an earlier formation, as per DATES). It's not directly ancestral to anything.
This is in line with the partial continuation of the Konstantinovka cultural layer on the Lower Don, as well as its Yhgs (mixed I-L704 and R-Z2103).
For years there has been a debate whether the agriculturalist ancestry in Yamnaya was from EEFs or from Transcaucasian people (or both, as I think is the case). Now that we have a sample such as KST001 with a profile very similar to Yamnaya in an early Maykop burial circa 3700 bc, does this help us get any closer to the answer? For the EEF proponents I think they need to provide a good explanation as to how this ancestry ended up in KST001 when we see little of it Ukraine at the time.
One interesting element here is that the model of Yamnaya as UKr_N-rich Sredny Stog + Remontnoye seems a bit less probable now as you had Yamnaya-like individuals with R-M269 living contemporary to the Remontnoye individuals. It also shows that rather than smooth clines the habitation was very patchy with clans moving about.
My best guess is that the relatives of KST001 circa 3500 bc started gaining demographic surpluses due to the utilization of the wagon and the exploitation of grassfields further away from water bodies. Over time this led to the replacement of the more diverse profiles developed in the earlier phase, and explains why Corded Ware, Yamnaya and Afanasievo are quite similar when compared to Cernavoda, Usatovo, North Caucasus steppe eneolithic, Remontnoye etc. The Steppe Maykop/Kumsay populations also benefited from this "revolution", leading tontheir appearance and interaction with the Caucasian sphere.
EthanR In my opinion you are making sense
Wouldn't Ustyurt culture make more sense than Kelteminar since it's more proximate to the area?
https://archives.palarch.nl/index.php/jae/article/download/1495/1514
@ Copper Axe
“ For the EEF proponents I think they need to provide a good explanation as to how this ancestry ended up in KST001 when we see little of it Ukraine at the time”
Circa 3700 the Ukraine eneolithic samples have tons of EEF ancestry (Cernavoda, some Sredni Stog)
But specifically for Yamnaya, someone would need to look at a more proximal approach than what’s posted here (which is appropriately distal to “triangulate” possible homeland scenarios), I might give that a go in a few days
There's probably both EEF and Anatolian-related Caucasus farmer ancestry in Yamnaya, but it's not much and not from one specific source that is potentially a good location for the PIE homeland.
Thus, the argument that the presence of this type of ancestry in Yamnaya puts the PIE homeland in Armenia or Iran is retarded, and that's the important point.
If the nucleus of the Yamnaya population lies in the Northwest Pontic steppe as archaeology and linguistics seems to suggest, then a good number of their ancestors must have been in close proximity to Trypillian and Balkan IE communities for centuries, just like they were with DDNC whom they have ancestry from. I know there’s been a concerted effort by certain academics to remove genetic/cultural ties of steppe people to the agricultural societies of “Old Europe” for ideological reasons, but the two spheres had been in contact on the north Black Sea since what, the late 5th millennium BC?
If Core Yamnaya do not require any or much surplus South Caucasus ancestry on top of what they can get from regular Steppe Eneolithic, then I don't understand why they need to be 20-25% “Aknashen” beyond persistent coping from people like Lazaridis.
@ David
What is your general idea of Yamnaya % wise?
@Davidski
I agree there's Anatolian-related Caucasus farmer ancestry in Yamnaya (through Nalchik-like admixture). What most people I guess are interested in, is whether this admixture was acquired already at the Srendy Stog stage (during core Yamnaya's formation) or after expanding throughout the PC Steppe when they hit a population boom.
Yamnaya in the North Caucasus definitely requires some Nalchik-like contribution (per G25), though many Yamnaya groups don't or trace amounts, so it seems like this admixture was unevenly spread and had a hard time leveling out because of the the higher population densities during their expansion, regardless of the higher mobility present on the steppe.
The "persistent coping" is because there are multiple lines of evidence now supporting it (in particular see the archeological description of Remontnoye found in the Lazaridis paper supplement).
@Copper Axe
I think KST001 is probably a recent migrant, whose profile did not form in the Kuban Steppe. It's just an exercise in making sense of regional substructures within the Sredni Stog sphere from here out. Unfortunately I'm not sure if we are going to get any terribly relevant samples, beyond the Vavilov paper, because of the conflict.
@Davidski
We fortunately don't hear too much about that anymore. We should be getting a lot of new Anatolian samples very soon, including quite a number of MBA Kultepe (Kanesh) samples.
I will be taking a close look at the language used in this thesis once it is accessible, because the thesis supervisor was one of the researchers working with those same Kultepe samples:
https://open.metu.edu.tr/handle/11511/113454
@Tom
What is your general idea of Yamnaya % wise?
I'm not 100% sure yet. Trying to work that out.
@EthanR
Do you think that Remontnoye was ancestral to Yamnaya in a significant way?
Or is it just the result of a related but largely parallel genetic and cultural development that is more Caucasus-shifted than the Sredny Stog ancestors of Yamnaya because it's located closer to the Caucasus?
@Davidski
I don't know. I lean against the south Caucasian ancestry in Sredni Stog/Yamnaya being the result of a sudden, singular pulse as opposed to something that happened over a longer time horizon due to exogemy etc.
This would be in line with the rough observation I made in an earlier thread that in Sredni Stog, South Caucasian ancestry seems to peak in the younger samples, regardless of other components.
Seems like, archeologically, the best link would be Svbodnoe or Remontnoye to facilitate this. The archeological description of Remontnoye provided by Anthony in the supplement notes a material culture with links to Sredni Stog, and a suggestion that they were seasonally migrating between the lower Don and the Manych Depression. Sounds like an ideal candidate, if accurate.
If something like that were the case, then Yamnaya would naturally be slightly more South Caucasian-rich than Sredni Stog due to being (a) slightly younger, and (b) originating from somewhere relatively east in the Sredni Stog cultural sphere, still in sufficient proximity to those marriage networks (such as Repin).
The Supplemental note for Remontnoye seems speculative. For ex: ''These mounds, usually solitary, were the earliest burials in the steppes north of the North Caucasus, and were distributed from the lower Kuban to the Caspian Depression. ', there is no comparative dating to support this statement.
Also the reference to migratory routes seem speculative, even if they had strontium data, i dont think it's very reliable
Not saying it is 'not correct', but it's not exactly proven
@ Rob
''Circa 3700 the Ukraine eneolithic samples have tons of EEF ancestry (Cernavoda, some Sredni Stog)'
Obviously Cernavoda are in western Ukraine. I suspect analysing all the Sredni Stog would be a major undertaking & challenging, we'd actually have to check if S/S have EEF.
This post says ''We do not know when the R1b-V1636 clans entered the northern Caucasus region, or from where''
But could R1b-V1636 be 'local' to the north Caucasus? Satanay was R1a-something, but that's just one sample.
I get the feeling that V1636 is somewhere half up the Volga
Aren't there Eneolithic EHG-like samples from north of the Caspian with R1b-V1636?
I think they generally all have 10-15% CHG. They are at Ekaterinovskiy and S'yezzheye.
They obviously have Berezhnovka-like ancestry and the clue is R-V1636.
All of these EHG groups in the forest steppe and even Karelia have a Berezhnovka-like signal to varying degrees. R-V1636 and J1 as well as the West Asian-related mtDNA clades attest to it.
Hunter-gatherers had low population densities. Contacts with hunter-gatherers from the steppe would cause geneflow that would be otherwise hard to dilute.
Doesn't mean that R-V1636 is local to the North Caucasus per se, but it's been there for quite a while at least.
@Davidski
Those EHG-like samples with R-V1636 date to the Neolithic.
They received their steppe ancestry well before ~ 6000 BCE.
It's the same basic admixture event as Khvalynsk, but where EHG ancestry wasn't as heavily diluted.
A mixture of a steppe population rich in CHG-related ancestry and a local forest steppe population poor in that ancestry.
Could you do these samples?
https://www.mediafire.com/file/gclchgzrglzm45e/Gyuris_2025.zip/file
I wonder if Steppe Maykop left a mark on the surrounding linguistic communities like NW Caucasian and PIE?
“ The "persistent coping" is because there are multiple lines of evidence now supporting it (in particular see the archeological”
The ~6% Aknashen ancestry possibly in Yamnaya is a far cry from the made-up Coping they came up with 🤣
Which R1b-V1636 individuals date to 6000 BC ?
Ekaterinovka Mis are c. 4500 BC (according to animal bones).
The Samara_HG is by contrast R1b-P297 ~ M73 (~ 5500 BC), with no CHG but a little bit of TTK.
This suggests that there was a migration from somewhere in the Caucasus-lower Volga-Caspian region toward the middle VOlga
This individual is interesting - Russia_Samara_Chekalino-4_N:I6303 BC_3578
Basically 100% EHG
As if Comb-Pick/ Lyalovo people replaced the 'cosmopolitan' Khvalysnk people before themselves being replaced by Yamnaya
@Rob
The earliest samples date to ~ 5500 BCE.
@ Moustaki
''The earliest samples date to ~ 5500 BCE.''
As per above, the corrosponding animal bones from the same burials only date c 4700 BC. Meaning 5500 is due to a several hundred year reservoir effect., suported by the isotopic dietary data.
Therefore we do not know where R1b-V1636 was c. 5500 bc
All I see is some non-carbon dated with a broad estimated range of 5500 - 4700 BC. You can tell the non dated samples, because they lack something like ''5925±25 BP, PSUAMS-8842'' after it
If Yamnaya emerged from the Lower Mikhaylovka (whose territory appears to be where most later Yamnaya kurgans, flat graves and signs of human activity also happen to be found), then archaeology suggests the “proto-Yamnaya” were originally a more sedentary, agro-pastoralist group wedged between and presumably in contact with Tripolye, EEF-rich Usatove, DDNC-rich cultures of the upper Dnieper, and Repin people. They wouldn’t have become nomadic pastoralists until after migrating to the Volga, Caspian, Kuban etc. from this comparatively western, “Old Europe” influenced homeland. I do not understand how this geography and time cannot adequately explain much of the Anatolia_N in Yamnaya.
Lazaridis claimed basal PIE was derived from a 50% Iran_Chl migrant wave that invaded EHG territory. That idea was then scrapped and we found out that the language was from CHG. Now the Yamnaya are 20-25% Aknashen_N. This is why I claim the man is “persistently coping”, because for years he’s been pushing this Ex Oriente Lux angle whilst regularly going on rants against Northern/Eastern Europe with the likes of Taleb on Twatter.
So do forgive me for being sceptical about his intentions and conclusions LOL.
@Norfern
https://drive.google.com/file/d/1Lqpw5QyoHzUYjJZ7Ws86-sVu7VplzsVQ/view?usp=sharing
Yamnaya potentially being from Mikhailovka I is not a problem, because Sredni Stog probably formed, and at the very least received ancestry from, further east.
But in any event, Yamnaya is probably from Repin.
Anyway, HF found the page for the Kultepe study:
https://search.trdizin.gov.tr/tr/yayin/detay/1259979/kultepe-kanis-toplulugunun-biyolojik-ve-sosyal-yapisinin-multidisipliner-bir-yaklasimla-analizi
"This project applies multidisciplinary bioarchaeological research, aiming to understand the complex and multilayered structures and dynamics of the Kültepe-Kanesh Bronze Age human population. Ancient DNA, biological distance, osteological, and stable carbon and nitrogen isotope analyses were applied to human skeletons from the Early and Middle Bronze Age. A total of 57 individuals were sampled for ancient DNA, and viable results were obtained from 29 samples. Population genetics analyses were carried out on 27 of these samples and kinship analyses on 13 samples. These results established that the sampled individuals from Karum Ib-Ia (1835-1685 BCE) and Early Bronze Age III levels are generally closer to Anatolian Bronze Age populations than to Mesopotamian and Syrian populations. Within the sampled individuals it was not possible to distinguish individuals genetically distinct from the others and possibly of Mesopotamian or Syrian origin. Kinship analyses have revealed that relationships are common, especially among individuals buried in the same grave type. For biological distance analysis, 102 non-metric dental characteristics were recorded from 44 individuals, and results compared with published data. These results demonstrated that the population is similar to populations of West Asia and North Africa, and closest to populations from Topaklı (Nevşehir) and Klazomenai (İzmir) in Anatolia.."
Repin ain't the origin, David Anthony rectified that theory last year or so. The thing with Repin is it clashes with Mikhailovka and is probably complementary with it as part of Post Sredny cultural complex. I don't remember precisely, but NV3003 sure isn't Yamnaya profile in autosomes, it's rather Sredny without Dnieper_N, i.e. an Eastern Sredny Don variant. KST001 is almost Yamnaya, albeit Maikop admixed given it's location lies in Vonyuchka. It seems some Remontnoye herders escaped to Sredny due to possible further intrusion of Maikop x Steppe Maikop westwards beyond Manych and into Ukraine proper.
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